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  3. Department of Biochemistry and Biophysics
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  5. Robert Daniels

Robert Daniels

Assistant Professor

Phone: +46 8 16 2460

E-mail:

Room: A 423


The maturation and asssembly of viral membrane glycoproteins.


Millions of people died world-wide as a result of the type A influenza pandemics of 1918 (H1N1), 1957 (H2N2) and 1968 (H3N2). The large number of avian influenza subtypes and the ability of influenza to undergo genetic reassortment and rapidly evolve raise the possibility that other pandemic strains can develop such as the pandemic H1N1 strain of swine origin in 2009 and the H7N9 strain in 2013. Currently, we cannot predict if all of the hemagglutinin (H) and neuraminidase (N) subtypes are capable of surfacing in the human population, but several factors (e.g. cell surface receptors, optimal replication temperatures, and mutations in the viral RNA polymerase) are known to act as barriers to genetic reassortment between human and avian influenza. Our lab is focused on investigating how the different subtypes of the influenza surface antigen neuraminidase assemble, mature and function under varying cellular and environmental conditions. This knowledge is then applied to the ever expanding influenza database to identify conserved and variable regions within and across subtypes to pinpoint features that are evolving or must evolve to cross the species barrier. Through cellular and biocheimcal analysis of how these alterations change neuraminidase and viral assembly and impact viral propagation, we can begin to illustrate a picture of the potential threats each subtype poses for genetic reassortment and the magnitude of the complimentary changes that are necessary for them to successfully enter the human population.


Selected Publications

  • Nordholm J, da Silva DV, Damjanovic J, Dou D, Daniels R. (2013).
    Polar residues and their positional context dictate the transmembrane domain interactions of influenza A neuraminidases.
    Journal of Biological Chemistry, 288(15):10652-60.
  • da Silva DV, Nordholm J, Madjo U, Pfeiffer A, Daniels R. (2012).
    “Assembly of subtype 1 influenza neuraminidase is driven by both the transmembrane and head domains.”
    Journal of Biological Chemistry, 288(1):644-53.
  • Mellroth P, Daniels R, Eberhardt A, Ronnlund D, Blom H, Widengren J, Normark S, Henriques-Normark B. (2012).
    “LytA, the major autolysin of Streptococcus pneumoniae, requires access to the nascent peptidoglycan.”
    Journal of Biological Chemistry, 287(14):11018-29
  • Daniels R, Mellroth P, Bernsel A, Neiers F, Normark S, von Heijne G, Henriques-Normark B. (2010).
    “Disulfide bond formation and cysteine exclusion in gram-positive bacteria.”
    Journal of Biological Chemistry, 285(5):3300-9.

Click "Publications" in the menu to the right to see the most recent publications.


Funding Sources

Swedish Foundation for Strategic Research through the Center for Biomembrane - Research
Swedish Research Council
Carl Trygger Foundation
Harald Jeanssons Stiftelse

Groupmembers


Candan Ariöz

Postdoc

+46 8 16 3148
A 427

Dan Dou

PhD Student

+46 8 16 3148
A 427

Johan Nordholm

PhD Student

+46 8 16 3148
A 427

Diogo da Silva

PhD Student

+46 8 16 3148
A 427

Mognad och hopsättning av virala membranglykoproteiner.


Miljoner av människor dog i världsomfattande influensa pandemier under åren 1918 (H1N1), 1957 (H2N2), och 1968 (H3N2). Det stora numret av underklasser av fågelburen influensa, tillsammans med influensans förmåga att genomgå genetisk omsortering och snabbt vidareutvecklas, ökar möjligheten till spontan framställning av andra, nya pandemiska stammar. För närvarande kan vi inte förutsäga om alla dessa underklasser är kapabla till att övergå till den mänskliga populationen, men flera faktorer (t.ex. receptorer på cellytan och optimal viral tillväxttemperatur) är kända för att agera som barriärer mot genetisk omsortering mellan human och fågelburen influensa. Vårt laboratorium fokuserar på att undersöka hur de olika underklassernas ytantigener hemagglutinin (HA) och neuraminidase (NA) sätts ihop, mognar och fungerar. Denna kunskap bör hjälpa oss att komma till insikt om möjliga hot som varje underklass utgör vad gäller genetisk omsortering och omfattningen av nödvändiga komplementära ändringar för överföring till den mänskliga populationen.

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