Structure and Mechanism of Solute Carrier (SLC) Transporters
Secondary transporters are the targets for many therapeutics and they often play a major role in drug pharmacokinetics. Understanding the mechanisms by which secondary transporters shuttle and move ions, drugs, and natural compounds across membranes is of fundamental importance. Because of the technical difficulties in working with membrane proteins our structural understanding is very limited. The goal of my research is to investigate the alternating access-mechanism of solute transporters which are critical to cell homeostasis and their dysfunction is associated with human diseases, such as cancer and cardiovascular heart disease. To achieve this we combine crystal structures with biochemical and biophysical techniques. These goals are facilitated by the development of novel methods in membrane protein overexpression, purification and crystallization.
Lee C, Kang HJ, von Ballmoos C, Newstead S, Uzdavinys P, Dotson D, Iwata S, Beckstein O, Cameron A.D, Drew D (2013)
A two-domain elevator mechanism for sodium/proton antiport.
Nature. Sept 26:501(7468):573-7.
Hu NJ, Iwata S, Cameron AD, Drew D (2011)
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.
Nature. Oct 3:478:408-11.
Sonoda Y, Newstead S, Hu N, Alguel Y, l Nji E, Beis K, Yashiro S, Lee C, Leung J, Cameron A.D, Byrne B, Iwata S, Drew D (2011).
Benchmarking membrane protein thermostability for improving throughput of high-resolution X-ray structures.
Structure. Jan 12:19(1):17-25.
Newstead, S., Kim H, von Heijne G, Iwata S, Drew D (2007)
High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in S. cerevisiae
Proc Natl Acad Sci U S A. Aug 28:104 (35):13936-41.
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